Plasma renin measurement is important in the diagnosis of the two most common forms of secondary hypertension which are Primary aldosteronism and Renovascular Hypertension. Plasma Renin evaluation can also help in selecting the most appropriate antihypertensive treatment.
The IDS-iSYS Direct Renin kit is based on chemiluminescence technology. Two monoclonal antibodies against renin are utilised. One is labelled with acridinium and the other with biotin.
Samples are incubated with both labelled antibodies along with buffer A and buffer B. Streptavidin coated magnetic particles are added and, following a further incubation step, the particles are captured using a magnet. After a washing step and addition of trigger reagents, the light emitted by the acridinium label is directly proportional to the concentration of Renin in the original sample.