Direct Renin

Product Description

The IDS-iSYS Direct Renin assay is intended for the quantitative determination of Direct Renin in human EDTA plasma on IDS Systems.

Results are to be used in conjunction with other clinical and laboratory data to assist the clinician in the assessment of hypertension related syndromes.

Key Features and Values

Unique Features

  • Highly specific monoclonal antibodies directed to the active renin enzyme site
  • Low cross-reactivity to Prorenin (<2.8%)
  • Referenced to the IS WHO 68/356
  • Time to first result is 43 minutes
  • Accurate and reliable patient results standardisation with the WHO IRP 68/356
  • Demonstrated clinical application with validated cut-off for the Aldosterone to Renin Ratio
  • Cost effective, process standardisation, long-term consistency

Scientific Description

Plasma renin measurement is important in the diagnosis of the two most common forms of secondary hypertension which are Primary aldosteronism and Renovascular Hypertension. Plasma Renin evaluation can also help in selecting the most appropriate antihypertensive treatment.

The IDS-iSYS Direct Renin kit is based on chemiluminescence technology. Two monoclonal antibodies against renin are utilised. One is labelled with acridinium and the other with biotin.

Samples are incubated with both labelled antibodies along with buffer A and buffer B. Streptavidin coated magnetic particles are added and, following a further incubation step, the particles are captured using a magnet. After a washing step and addition of trigger reagents, the light emitted by the acridinium label is directly proportional to the concentration of Renin in the original sample.

Complementary Products

IDS-iSYS Direct Aldosterone

Publications

Publications using the IDS-iSYS Direct Renin assay

  1. Genovesi S, Antolini L, Orlando A, Tassistro E, Giussani M, Nava E, Turolo L, Manolopoulou J, Parati G, Morganti A. Aldosterone-to-renin ratio depends on age and sex in children attending a clinic for cardiovascular risk assessment. J Hypertens. 2018 Feb;36(2):344-352.
  2. O’Shea PM, Griffin TP, Browne GA, Gallagher N, Brady JJ, Dennedy MC, Bell M, Wall D, Fitzgibbon M. Screening for primary aldosteronism using the newly developed IDS-iSYS® automated assay system. Pract Lab Med. 2016 Nov 13;7:6-14.
  3. O’Shea P, Brady JJ, Gallagher N, Dennedy MC, Fitzgibbon M. Establishment of reference intervals for aldosterone and renin in a Caucasian population using the newly developed Immunodiagnostic Systems specialty immunoassay automated system. Ann Clin Biochem. 2016 May;53(Pt 3):390-8.
  4. Manolopoulou J, Fischer E, Dietz A, Diederich S, Holmes D, Junnila R. Clinical validation for the aldosterone-to-renin ratio and aldosterone suppression testing using simultaneous fully automated chemiluminescence immunoassays. J. Hypertens. 2015;33:2500–2511.
Product Code: IS-3400/ IS-3430 CE Marked
IS-4800/ IS-4830/ IS-4835 FDA Approved
Certification:

CE Marked
FDA Cleared

Clinical Area: Endocrine Hypertension (Endocrinology)
Type: Automated
Format: Automated Chemiluminescence Immunoassay (CLIA)
RUO/IVD: IVD
Number of Tests: 100
Sample Type: EDTA plasma
Sample Volume: 190 µL + dead volume
Assay Range: CE Marked Assay 1.8 - 550 μIU/mL
FDA Marked Assay 2.0 - 256 pg/mL

Need more information? Get in touch

Fill in the boxes below to get in contact